GlowCas9: A CRISPR Protein That Lights Up Gene Editing

In December 2025, scientists at the Bose Institute, Kolkata, developed GlowCas9, a novel CRISPR protein that emits light while performing gene editing.

What makes GlowCas9 special?

GlowCas9 introduces a new way to visualise genome editing as it happens:

• It is a bioluminescent version of Cas9, created by fusing Cas9 with a split nano-luciferase enzyme derived from deep-sea shrimp proteins.
• When Cas9 folds correctly inside a cell, the enzyme pieces rejoin and emit light, similar to a gentle firefly glow.
• This enables scientists to track CRISPR activity in living cells, tissues, and even plant leaves, without destroying them.

Why This Discovery ....